High Purity Exosome Extraction Kit (Cell Culture Supernatant，4ml/T)

1. If precipitation occurs at low temperatures，dissolve it briefly in a 37°C water bath，shake well，and use. 2. Use the reagent promptly after opening. 3. Avoid allowing the filter membrane in the centrifuge tube to dry once moistened. If not used immediately after pre-washing，keep the liquid on the filter membrane until use. High Purity Exosome Extraction Kit (Cell Culture Supernatant) is suitable for extracting high-purity total exosomes from various primary or passaged cell culture supernatant samples. No ultracentrifugation is required; a large quantity of structurally intact exosomes can be obtained from the sample through simple routine centrifugation. This method is characterized by its rapid simplicity and high extraction efficiency，saving significant experimental time and sample resources. The extracted exosomes can be utilized for diverse downstream applications based on experimental objectives，offering broad applicability. The conventional method for isolating and extracting exosomes from in vitro cultured cells is ultracentrifugation. This approach is time-consuming and labor-intensive，typically requiring 30-70 hours per run，processing a maximum of six samples at a time，demanding substantial starting material，and yielding low output. This kit enables rapid，high-efficiency isolation of high-purity exosome particles through simple centrifugation. The extracted exosomes remain intact and suitable for diverse downstream applications including functional studies，protein and RNA analysis，electron microscopy，and NTA particle size analysis. This kit is designed to process 4 ml of cell culture supernatant per sample. Each 50T kit can extract approximately 200 ml of cell culture supernatant. For large-scale processing，dilute the extraction solution 1:4 with cell culture supernatant based on the total volume. Applicable Samples: Cell culture supernatant.