Hexokinase(HK) Activity Assay Kit

Determination of Significance: Hexokinase (HK, EC 2.7.1.1) is widely distributed in animals, plants, microorganisms and cultured cells. It is the first key enzyme in the process of glucose decomposition, catalyzing the conversion of glucose into glucose 6-phosphate, which is the intersection of glycolysis and pentose phosphate pathways. Measurement Principle: HK catalyzes the synthesis of glucose to 6-phosphate glucose, and 6-phosphate glucose dehydrogenase further catalyzes the dehydrogenation of 6-phosphate glucose to NADPH, which has a characteristic absorption peak at 340 nm. Self Provided: Spectrophotometer/microplate reader, water-bath, table centrifuge, adjustable pipette, micro quartz cuvette/96 well flat-bottom plate (UV plate), mortar/homogenizer and ice. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.