HE Agar

1.Due to varying microwave power, if the medium is not fully melted within 3 minutes, crush it into pieces before heating on high power, or extend the heating time by 0.5 to 1 minute.
2.Check for contamination. Do not use if microbial growth is observed.
3.Pour plates under aseptic conditions to prevent microbial contamination.
4.Do not overheat in the microwave, as excessive heating will cause severe water loss and reduce the nutritional performance of the product.
5.This product is for single use only. Pour out all the medium in the bottle and avoid repeated melting and solidification.
6.This product is not intended for clinical diagnosis. Test Principle: Peptone and beef extract provide carbon sources, nitrogen sources, vitamins and minerals. Lactose, sucrose and salicin are fermentable sugars. Bile salts and sodium deoxycholate inhibit the growth of Gram-positive bacteria. Sodium chloride maintains osmotic balance. Sodium thiosulfate and ammonium ferric citrate detect hydrogen sulfide production, turning the colony centers black. Agar serves as the gelling agent of the medium. Bromothymol blue and acid fuchsin act as pH indicators. Colonies that ferment sugars to produce acid appear orange-yellow, while non-fermenting colonies show blue-green color.