Glycosylated Serum Protein Assay Kit（ NBT-Colorimetric Method）

Determination of Significance: Serum glucose reacts non-enzymatically with the N-terminal amino group of albumin and other serum protein molecules to form a polymer ketamine structure. Under alkaline conditions, it reacts with nitrotetrachloroazole blue to produce formazan, a purple-red compound. Comparing the color at 530 nm wavelength, measuring its OD value. And comparing with DMF standard, the content can be obtained. Measurement Principle: Serum glucose reacts non-enzymatically with the N-terminal amino group of albumin and other serum protein molecules to form a polymer ketamine structure. Under alkaline conditions, it reacts with nitrotetrachloroazole blue to produce formazan, a purple-red compound. Comparing the color at 530 nm wavelength, measuring its OD value. And comparing with DMF standard, the content can be obtained. Self Provided: Spectrophotometer, desk centrifuge, transferpettor, 1 mL glass cuvette, mortar/homogeniser, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.