Glycerol-3-Phosphate Oxidase Activity Assay Kit

Glycerol-3-phosphate oxidase (GPO, EC 1.1.3.21) is an enzyme present in the cytoplasm, mainly involved in a part of the fat metabolism pathway, known as the glycerol phosphate pathway. Glycerol phosphate oxidase (GPO) oxidizes glycerol phosphate to dihydroxyacetone phosphate and produces hydrogen peroxide. Under the action of α-glycerophosphate dehydrogenase, NADH and dihydroxyacetone phosphate produce α-glycerophosphate and NAD+, causing a decrease in absorbance at 340nm. The activity of glycerol-3-phosphate oxidase can be calculated by measuring the change of NADH.