Glutamine (Gln) Content Assay Kit

Determination of Significance: Glutamine is the amide of glutamic acid and one of the important amino acids that make up proteins. At the same time, glutamine is also the main source of α-ketoglutaric acid in the tricarboxylic acid cycle. Glutamine exists in the organism in two states: free state and bound state. Free glutamine plays an important role in organism metabolism, and its metabolism accounts for more than 60% of free amino acids in cells and blood circulation. Measurement Principle: Free glutamine is converted to glutamic acid under the catalysis of glutaminase, Under the catalysis of glutamate dehydrogenase (GDH), glutamate and NAD are produced α- Ketoglutaric acid, NADH and NH4+, under the action of 1-mPMS, WST can react with NADH to produce water-soluble formazan, which has the maximum absorption peak at 450 nm, and the glutamine content can be calculated. Self Provided: Microplate Reader/Spectrophotometer, Desktop Centrifuge, Water Bath/Constant Temperature Incubator, Transferpettor, Mortar/Homogenizer/Cell Ultrasonic Crusher, Micro Glass Cuvette/96 Well Flat-bottom Plate, Chloroform and Distilled Water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.