Glutamate Synthase(GOGAT) Activity Assay Kit

Determination of Significance: Glutamine oxoglutarate aminotransferase (also known as Glutamate synthase) is an enzyme and frequently abbreviated as GOGAT. GOGAT is mainly found in the protoplasts of prokaryotes, yeasts and non-green tissues of higher plants. Together with glutamine synthetase (GS), it constitutes the GS/GOGAT cycle and participates in the regulation of ammonia assimilation. Measurement Principle: GOGAT uses NADH as an electron donor to catalyze the amino transfer of glutamine to α-ketoglutarate to form two molecules of glutamic acid. The activity of GOGAT can be determined by the decrease rate of NADH at 340 nm. Self Provided: Microplate reader/spectrophotometer, desk centrifuge, adjustable transferpettor, water bath, micro quartz cuvette/96 well UV flat-bottom plate, mortar/ homogenizer, ice, distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.