Fructose-1，6-diphosphate(FDP)Content Assay Kit

Measurement significance: Fructose-1,6-Diphosphate (FDP) is an important intermediate product in the process of glycolysis. It has a regulatory effect on a variety of enzymes,and can improve cell energy metabolism,increase energy utilization anti-arrhythmia and anti-tissue peroxidation. It is widely used in clinical medicine. Determination method: Aldolase catalyzes fructose 1,6-diphosphate to produce glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. Under the action of triose phosphate isomerase and α-glycerophosphate dehydrogenase,NADH and dihydroxyacetone phosphate are catalyzed to produce NAD+ and α-glycerophosphate, causing a decrease in absorbance at 340 nm. The content of fructose 1,6-diphosphate can be calculated by measuring the change of NADH. Instruments and supplies to be provided: Spectrophotometer/Microplate Reader,balance,low temperature desktop centrifuge,water bath/thermostatic incubator,1mL quartz cuvette,adjustable pipette,mortar/homogenizer / cell ultrasonic disruptor,vortex oscillator,ice,distilled water and EP tube. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.