Free Fatty Acid (FFA) Content Assay Kit (Copper Soap Method)

Free fatty acids (FFAs) refer to non-esterified fatty acids. In serum ， FFAs primarily serve as intermediate products of fat metabolism and are crucial metabolic substrates for cellular energy metabolism ， providing energy for organs such as the heart ， liver ， and skeletal muscle. Free fatty acid testing holds significant clinical importance ， as it can be used to assess an organism’s metabolic state and disease risk. This assay kit is suitable for determining free fatty acids in animal tissues ， plant tissues ， edible oils ， and other samples. Free fatty acids in the sample dissolve in hydrocarbon solvents and react with a pyridine-copper ion colorimetric solution to form copper soap complexes. These complexes exhibit a characteristic absorption peak at 710 nm ， enabling the calculation of FFA content.