Fatty Acid Synthase(FAS) Activity Assay Kit

Determination of Significance: Invertase (Ivr) catalyzes the irreversible decomposition of sucrose into fructose and glucose, which is one of the key enzymes in sucrose metabolism of higher plants. According to the optimal pH, Ivr can be divided into two types: acid invertase (AI) and neutral invertase (Ni). AI (EC 3.2.1.26) mainly exists in cell vacuole or free space, and the optimal pH is 4.5-5.0 (acid). It can regulate the utilization of sucrose in vacuole and the accumulation of sugar in fruit by degrading sucrose in vacuole. Measurement Principle: AI catalyzes the degradation of sucrose to produce reducing sugar, and further reacts with 3,5-dinitrosalicylic acid to form brownish red amino compound, which has a characteristic light absorption at 540 nm, and the increase rate of light absorption at 540 nm in a certain range is in direct proportion to AI activity. Self Provided: Spectrophotometer/microplate reader, desktop centrifuge, water-bath, adjustable pipette, micro glass cuvette/ 96 well flat-bottom plate, mortar/homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.