Exosome Rapid Extraction Kit (Cell Culture Supernatant，4ml/T)

1. If precipitation occurs at low temperatures，dissolve it briefly in a 37°C water bath，shake well，and use. 2. Use the reagent promptly after opening. 3. Avoid allowing the filter membrane in the centrifuge tube to dry once moistened. If not used immediately after pre-washing，keep the liquid on the filter membrane until use. Exosome Extraction Kit (Cell Culture Supernatant) is suitable for isolating total exosomes from various cell culture supernatant samples. It eliminates the need for ultracentrifugation，enabling the recovery of large quantities of structurally intact exosomes from samples through simple routine centrifugation. Characterized by rapid，straightforward procedures and high extraction efficiency，it saves significant experimental time and sample resources. The extracted exosomes can be utilized for diverse subsequent experiments based on research objectives，offering broad applicability. Traditional methods for isolating and extracting exosomes from cell culture supernatants primarily rely on ultracentrifugation. This approach is time-consuming and labor-intensive，often requiring 30-70 hours per run，processing a maximum of six samples at a time，demanding substantial starting material，and yielding low output. This kit enables rapid，high-efficiency isolation of high-purity exosome particles through simple centrifugation. The extracted exosomes remain intact and suitable for diverse downstream applications including functional studies，protein analysis，and RNA analysis. Based on processing 4 ml of cell culture supernatant per sample，each 50 T kit can process approximately 200 ml of cell culture supernatant. Applicable Samples: Cell culture supernatant.