Eosin Methylene Blue Agar

1.Due to varying microwave power, if the medium is not fully melted within 3 minutes, crush it into pieces before heating on high power, or extend the heating time by 0.5 to 1 minute.
2.Check for contamination. Do not use if microbial growth is observed.
3.Pour plates under aseptic conditions to prevent microbial contamination.
4.Do not overheat in the microwave, as excessive heating will cause severe water loss and reduce the nutritional performance of the product.
5.This product is for single use only. Pour out all the medium in the bottle and avoid repeated melting and solidification.
6.This product is not intended for clinical diagnosis. Test Principle: Peptone provides carbon and nitrogen sources. Lactose is a fermentable sugar for coliforms. Dipotassium hydrogen phosphate acts as a buffer, and agar serves as the medium gelling agent. Eosin and methylene blue function as bacteriostatic agents and pH indicators. They inhibit Gram-positive bacteria and form precipitates under acidic conditions, resulting in purplish-black colonies or colorless transparent colonies with black centers.