Enhanced Cell-Free Protein Expression Kit (Disulfide Ehhanced Version)

This product can express conventional proteins and is particularly suitable for expressing proteins containing disulfide bonds. This product is based on E. coli expression system, employing a precisely prepared cell lysate that fully preserves ribosomal complexes, translation factors, and core enzyme activity. It enables the perfect in vitro reconstruction of a biosynthetic factory, achieving rapid “one-stop” conversion from DNA/RNA templates to functional proteins. The system employs a T7 promoter system, supporting diverse template inputs including circular plasmid DNA, linear DNA, and mRNA. By utilizing active components such as ribosomes, translation factors, and enzymes from the cell lysate, while supplementing energy, nucleotides, amino acids, and inorganic salts, it reconstructs the transcription-translation system in vitro. Using DNA or RNA as templates, it expresses proteins. Cell-free protein expression is a method independent of live bacteria, enabling rapid, flexible, and large-scale protein expression. Compared to traditional recombinant expression systems, it offers numerous advantages: 1. Rapid protein expression: Target proteins are expressed within 1-2 hours, reaching maximum levels within 8-24 hours. 2. High protein yield: Maximum protein concentrations exceeding 2 mg/ml can be achieved. 3. Simple and flexible reactions: Requires only the addition of DNA or RNA templates to the reaction system, compatible with 96-well plates or centrifuge tubes.