Endo-Free High-Purity Plasmid Maxi Rapid Isolation Kit

1. Add the entire supplied RNase A to Solution P1 before use，mix well and store the mixture at 2–8 °C. 2. Check Solution P2 and P4 for crystals or precipitates prior to use. If any are observed，warm the solutions in a 37 °C water bath for several minutes until they become clear.3. Avoid direct contact with Solution P2 and P4. Tighten the caps immediately after use. 4. Pull the plunger out of the filter tube slowly and carefully when using the filter，to prevent the filter membrane from loosening due to pressure. 5. Plasmid yield is related to bacterial culture density，plasmid copy number and other factors. For low-copy plasmids or large plasmids larger than 10 kb，increase the amount of bacterial pellet and proportionally raise the volume of Solution P1，P2 and P4. Preheat the elution buffer in a 65-70 °C water bath. Appropriately extend the binding and elution time to improve extraction efficiency. This kit utilizes proprietary silica membrane adsorption technology to bind plasmid DNA efficiently and specifically. Specially formulated Solution P4 and Filter CS1 effectively remove endotoxins，proteins and other contaminants. The entire extraction process is simple and time-saving，completed within just 1 hour. Plasmid DNA isolated with this kit is compatible with routine experiments，including restriction digestion，PCR，DNA sequencing，ligation，transformation and cell transfection. Recommended culture volume per preparation: 100 mL for high-copy plasmids with a typical yield of 500-1500 μg; 200 mL for low-copy plasmids with a typical yield of 200-600 μg. Product Features: No toxic reagents such as phenol or chloroform，and no ethanol precipitation are required. High-quality high-copy plasmids (0. 2-0. 4 mg) can be rapidly extracted from 100-200 mL of E. coli LB culture medium，with a recovery rate of 80-90%. The obtained plasmids deliver high yield，high supercoiled fraction and excellent purity，and can be directly applied to various molecular biology experiments，such as restriction digestion，transformation，PCR，in vitro transcription，sequencing and transfection.