Dark Red Cell Cycle Analysis Solution

The regulatory system of the cell cycle is closely related to cell proliferation. Cell division in which one cell produces two daughter cells is part of the cell cycle. The cell cycle can be analyzed by measuring cell DNA staining by flow cytometry and then analyzing the proportion of stained nuclei during the cell cycle phase. The cell cycle is divided into two main phases: interphase and mitotic (M) phase. Interphase consists of G1 phase (preparation for DNA synthesis that occurs before cell division)，S phase (DNA synthesis and replication)，and G2 phase (single cell with repetitive chromosomes). M phase includes mitosis and cytokinesis. Cell cycle progression is regulated by cyclins and cyclin-dependent kinases. Analyzing the mechanism of action of cell cycle checkpoints is of great significance for determining the role of anticancer drugs and other drugs. The cell cycle control mechanism is closely related to cell proliferation. The process of cell division in which one cell divides to produce two daughter cells is also done through the cell cycle. Flow cytometry is widely used for cell cycle analysis，and by staining DNA with fluorescent dyes and measuring with flow cytometry，the ratio of interphase and M phase can be calculated. Among them，propidium iodide (PI) is one of the most widely used dyes for the analysis of the cell cycle. However，when measuring the cell cycle with PI，the following pretreatment is required: 1. Cell fixation (PI has no cell membrane permeability); 2. RNase treatment (PI has no DNA selectivity，RNA will also be stained); 3. Compensation (fluorescence compensation is required when using PE and FITC filters for co-staining). This product is a product that does not require cell fixation and RNase treatment，and can be performed with simple operation for cell cycle analysis. In addition，it is a preparation-free，ready-to-use aqueous solution with two detection fluorescence wavelengths，Deep Red and Blue，which can be freely selected according to the experimental needs and the equipment used. And it can also be applied to immobilized cells.