Creatine Kinase(CK) Activity Assay Kit

Determination of Significance: Creatine kinase (CK) (EC 2.7.3.2) is also known as creatine phosphokinase, which mainly exists in heart, muscle and brain. It can reversibly catalyze the trans-phosphoryl reaction between creatine and ATP. It is an important kinase directly related to cell energy transport, muscle contraction and ATP regeneration. Measurement Principle: CK catalyzes creatine phosphate and ADP to generate creatine and ATP, hexokinase catalyzes ATP and glucose to generate glucose-6-phosphate, and glucose-6-phosphate dehydrogenase catalyzes glucose-6-phosphate and NADP+ to generate NADPH, resulting in an increase of 340 nm light absorption value, which is used to express CK enzyme activity. Self Provided: Scales, low temperature centrifuge, constant temperature water bath, spectrophotometer/microplate reader, micro quartz cuvette/96 wellflat-bottom(UV) plate and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.