Citrate Synthase(CS) Activity Assay Kit

Determination of Significance: Citrate Synthase (CS, EC 2.3.3.1) is widely exists in animals, plants, microorganism and mitochondrial matrix of cultured cells. It is the first rate-limiting enzyme in the tricarboxylic acid cycle and one of the main regulatory sites. Measurement Principle: CS catalyzes acetyl CoA and acetoacetic acid to generate citryl coenzyme A, further hydrolysis to produce citric acid. The reaction promoted the transformation of colorless DTNB to yellow TNB, which has absorption at 412 nm. Self Provided: Spectrophotometer, low temperature centrifuge, water bath, mortar/homogenizer, adjustable pipette, 1 mL glass cuvette and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.