CHO Transfection Reagent and Protein Expression System II (Suspension Culture without Serum)

CHO cell lines are currently one of the most widely used host cells for recombinant protein expression. CHO cells can be cultured either as adherent cells or in suspension. Different culture methods require distinct media and specific plasmid/DNA transfection reagents. Despite the increasing application of CHO cells in recombinant protein expression，there remains a lack of highly efficient CHO plasmid transfection and expression systems in domestic market. Particularly for suspension-cultured CHO cells，plasmid transfection and protein expression outcomes remain suboptimal. CHO Transfection Reagent and Protein Expression System I is primarily designed for transfection and expression in adherent CHO cells using conventional serum-containing media. CHO Transfection Reagent and Protein Expression System II is specifically formulated for transfection and expression in suspension-cultured CHO cells using serum-free media. Features: 1. Specially formulated serum-free culture system supports high-density suspension culture of CHO cells，particularly suitable for large-scale expression and preparation of recombinant proteins; 2. Proprietary high-efficiency CHO transfection reagent achieves up to 70% transfection efficiency in CHO cells，with protein expression levels approximately 20% higher than non-upgraded CHO expression systems; 3. Simplified workflow: Recombinant plasmid transfection can be performed directly in suspension medium，eliminating the need for initial transfection in conventional medium followed by conversion to suspension culture; 4. Comprehensive kit: The expression system includes serum-free medium，CHO-specific transfection reagent，Trans buffer，and culture supplements，fully meeting the requirements of most users.