کیت
Solarbio

Chicken IgG ELISA Kit

توضیحات و پروتکل‌های فنی

Solarbio ELISA kit uses the quantitative sandwich enzyme immunoassay technique. An antibody specific for chicken IgG has been pre-coated onto a microplate; Add gradient-diluted standards and pre-diluted samples separately and allow them to fully combine with the specific antibody. After washing away any unbound substances a horseradish peroxidase (HRP)-conjugated antibody specific for chicken IgG is added to detect the captured chicken IgG protein in the sample. After extensive washing a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. A Solution containing sulfuric acid is used to stop color development. The color intensity proportional to the quantity of bound protein is then measurable at 450nm. You can calculate the concentration of chicken IgG in the samples by comparing the O.D. of the samples to the standard curve.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKCN-0126-48T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃ 6 months
Assay Time:
2.75h
Reactivity:
Chicken
Detection Range:
0.5-32 ng/ml
Recovery:
80%-120%
Sample Type:
Serum Plasma Cell Culture Supernatant
Precision:
Both intra-plate and inter-plate variation coefficients were less than 10%
Sensitivity:
250 pg/ml
Specificity:
Do not react with Chicken IgM IgA duck IgG, goose IgG Human IgG Mouse IgG Rat IgG OVA etc.
تحویل:
یک ماه پس از سفارش
100200000
100200000 تومان