CCK-8 Cell Proliferation and Cytotoxicity Assay Kit

CFDA-SE cell proliferation and tracer detection kit is based on CFDA-SE for cell tracer and proliferation detection kit. The main working principle of the kit is that CFDA-SE is a fluorescein dye with membrane permeability and does not have fluorescence luminescence. When it enters the living cell through the cell membrane, it can be catalyzed by the esterase in the cytoplasm to produce carboxyfluorescein succinimide (carboxyfluorescein succinimidyl ester, CFSE), which can emit strong green fluorescence, can not penetrate the cell membrane, and can be well retained in the cell. CFSE can also spontaneously and irreversibly bind to intracellular amino groups and then couple to cellular proteins. At the same time, excess and uncoupled CFDA-SE is passively diffused back into the extracellular medium and removed by subsequent cleaning steps. The fluorescence of non-mitotic cells labeled by CFDA-SE is stable, and the labeling time can be up to several months, so it is suitable for cell community analysis. The fluorescence of CFDA-SE-labeled cells is uniform, during cell division and proliferation, CFSE-abeled fluorescence can be evenly distributed among the two offspring cells, and the fluorescence intensity becomes half of that of parent cells. The number of cell divisions can be detected by flow cytometry (FL1 channel) depending on the fluorescence intensity, detecting undividing cells, divided once (1/2 fluorescence intensity), twice (1/4 fluorescence intensity), three times (1/8 fluorescence intensity), and more divisions of cells. CFDA-SE can detect cell divisions for up to eight or even more divisions. CFDA-SE-labeled cells can be used to study the proliferation of cells in vitro and in vivo, and have the function of not staining adjacent cells. CFDA-SE is most commonly used to detect the proliferation of lymphocytes and can also be used to detect the proliferation of fibroblasts, natural killer cells, hematopoietic progenitor cells and other cells. CFDA-SE-labeled cells showed green fluorescence, Ex=494 nm, Em=521 nm, and FL1 detectionchannel could be used when flow cytometry was used. CFDA-SE-labeled cells can also be observed by fluorescence microscope. It can be detected by 488 nm excitation wavelength. This product is a CFDA-SE cell proliferation and tracer detection kit, which contains the solvent needed for the preparation of CFDA-SE storage solution and the staining buffer for cell labeling, which simplifies the preparatory work of the experiment. CFDA-SE labeling of cells can be completed in 10-15 min. For different cells, it is necessary to explore the best labeling time. According to the fluorescent probe labeling volume of each sample is calculated as 1 mL, the two specifications of this kit can be detected 1000 times and 2000 times respectively. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.