Caspase-1 Activity Assay Kit

Determination of Significance: Caspase is a family of proteases involved in the process of apoptosis, including more than 10 members. Caspase-1 is the only caspase that can cleave IL-1b and IL-18 precursors to produce active cytokines. Caspase-1 regulates apoptosis by cleaving BCL XL, and regulates cytokine mediated immune-inflammatory response by cleaving cytokine precursors. Measurement Principle: The caspase-1 colorimetric assay is based on the hydrolysis of the peptide substrate Tyr-Val-Ala-Asp-p-nitroanilide (YVAD-pNA) by caspase-1, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405 nm. The activity of Caspase can be calculated by detecting pNA. This kit is suitable for mammalian tissue and cell. Self Provided: Spectrophotometer/Microplate reader, 100μL cuvette/ 96-well plate, centrifuge, water bath / incubator, adjustable pipette, mortar / homogenizer, ice and distilled water Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.