Carbonic Anhydrase Activity Assay Kit

Determination of Significance: Carbonic anhydrase (CA, EC4.2.1.1) is a metalloenzyme with Zn 2+ as the active center, which can efficiently catalyze the reversible hydration reaction of CO2: CO2+H2O ?HCO3-+H+ at a rate 107 times higher than that under natural conditions, which is one of the fastest known enzymes. It is one of the fastest known enzymes. Measurement Principle: Carbonic anhydrase can catalyze the reaction of p-nitrophenyl acetate to form p-nitrophenol, and the rate of increase of absorbance value at 405 nm reflects the activity of carbonic anhydrase. Self Provided: Spectrophotometer/microplate reader, centrifuge, water bath/incubator, adjustable pipette, Micro glass cuvettes/96 well plates , mortar/homogenizer/cell sonicator, ice and distilled water. . Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.