Burkholderia Cepacia Selective Agar

Principle: Casein peptone and yeast extract powder provide the carbon and nitrogen sources, vitamins and growth factors required for microbial growth; Lactose and sucrose are fermentable sugars; Sodium chloride maintains a balanced osmotic pressure; Phenol red is an acid-base indicator; Crystal violet inhibits the growth of gram-positive bacteria; Agar is used as a coagulant for the medium. Gentamicin is a broad-spectrum antibiotic with strong antibacterial activity against most Pseudomonas, Proteus, coliforms, staphylococcus, aerobic bacillus and other Gram negative and negative bacteria; Vancomycin can inhibit the synthesis of bacterial cell wall, has a strong effect on Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, etc. , and also has a good effect on Clostridium difficile, Bacillus anthracis, diphtheria bacillus, etc. ; Polymyxin B has inhibitory effects on a variety of gram-negative bacteria such as Pseudomonas aeruginosa, Escherichia coli, Klebsiella and Haemophilus. Usage: Weigh 50. 58g of this product, heat and boil to completely dissolve it in 1000ml distilled water, divide into triangular bottles, 200ml per bottle, and autoclave at 121℃ for 15 minutes. When cooling to 45℃-50℃, add 1 Gentamicin Supplement (2 mg), 1 Vancomycin Supplement (0. 5 mg) and 1 PolymyxinB Supplement (120,000U) per 200 ml of medium, mix well, pour into a sterile dish, and set aside.