Brucella Medium Base

Preparation Method: Weigh 45. 0 g of this product in 1L distilled or deionized water, heat and boil until completely dissolved, subpack, autoclave at 121℃ for 15 minutes, cool to about 50-55℃, add 5% inactivated horse serum per 100ml medium (keep warm at 56℃ for 30 minutes). And one Brucella selective additive or one modified Brucella selective additive, mix, pour plate, set aside. Composition: g/L Peptone: 10. 0 Sodium chloride: 5. 0 Agar: 15. 0 Beef infusion powder: 5. 0 Glucose: 10. 0 pH: 7. 5±0. 2, 25℃ Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.