Solarbio

Bovine E2 ELISA Kit

توضیحات و پروتکل‌های فنی

Solarbio ELISA kit uses the quantitative competitive enzyme immunoassay (competitive ELISA) technique. An bovine E2 antigen has been pre-coated onto a microplate; Add gradient-diluted standards and pre-diluted samples separately then add a HRP-conjugated antibody specific for bovine E2 to allow competitive binding between soluble bovine E2 in the samples/standards and the plate-coated bovine E2 for the limited antibody binding sites. After extensive washing a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. A Solution containing sulfuric acid is used to stop color development. The color intensity inversely proportional to the concentration of bovine E2 is then measurable at 450nm. You can calculate the concentration of bovine E2 in the samples by comparing the O.D. of the samples to the standard curve.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKB-0368-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

1h30min

Reactivity:

Bovine

Detection Range:

15.625-1000 pg/ml

Recovery:

80%-120%

Sample Type:

Serum Plasma Cell Culture Supernatant

Precision:

Both intra-plate and inter-plate variation coefficients were less than 10%

Sensitivity:

5.87 pg/ml

Specificity:

Can detect cow E2 in the sample, and there is no significant crossover with other related proteins.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

130200000 تومان