BMGY/BMMY Medium Base

Product Introduction: YNB and biotin were added to induce expression in Pichia pastoris, and 1% glycerol or 0. 5% methanol was added during induction. Preparation Method: Weigh 44. 1g of this product, boil and dissolve it in 800ml deionized water, autoclaving at 121℃ for 15 minutes, cooling and aseptic operation, add 100ml of 13. 4%YNB solution and 2ml of 0. 02% biotin solution. Before induction, BMMY medium was prepared by adding 100ml 5% methanol aqueous solution filtered for sterilization, and BMGY medium was prepared by adding 100ml sterile 10% glycerol aqueous solution. Composition: g/L Peptone: 20. 0 Yeast Infusion powder: 10. 0 pH6. 0 Phosphate buffer: 100mmol Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.