Blue-Sepharose Cl-6B

Description: Separation reagent, chromatographic medium. Product Introduction Blue agarose gel 6FF is the coupling of blue dye to highly coupled agarose gel, which has the characteristics of chemical stability, high mechanical strength and fast flow rate. Mainly through the combination of electron-pair action and hydrophobic action with the target protein, a very widely used affinity chromatography material, suitable for the industrial purification of albumin, interferon, α-2 macroglobulin, coagulation factors and various enzymes requiring NAD+ and NADP+. Product parameters Substrate: 6% crosslinked agarose gel Ligand: Blue dye Ligand density: 10μmol/mL Particle size: 45-165μm Maximum flow rate: 300cm/h Working PH: 3-10 Cleaning PH: 2-13 Operating temperature: room temperature Storage condition: Stored in 20% ethanol at +4-8℃ How to use 1. Install the column First, the gel and the reagent used are taken out of the refrigerator in advance, returned to room temperature, and the required amount of gel is taken according to the size of the column (about 1. 15 times the volume of the column bed), and the gel preservation liquid 20% ethanol is removed by cleaning, and then drained. Then moisten the column with ultra-pure water or buffer, and retain some liquid, pour all the gel homogenate into the column along the glass rod to ensure that there are no bubbles in the column, open the lower end of the column outlet, let the gel settle naturally, and then balance the column with buffer; Step 2 Balance Run the buffer through the column at a constant flow rate, balancing 5-10 column bed volumes until the effluent conductance and pH are constant. 3. Sample delivery The sample volume is about 1-2% of the column volume, the sample is prepared with buffer, and the turbid sample is centrifuged and filtered before the sample is fed. Step 4 Elution Eluting with buffer, keep the flow rate and buffer composition unchanged during elution. Step 5 Regenerate 5 column bed volumes were washed in turn with ultra-pure water and 20% ethanol, and the columns were stored in 20% ethanol at +4-8℃. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.