BCSA Enrichment Broth with Gentamycin

Principle: Casein peptone, yeast extract provides basic nutrients for bacterial growth, sodium chloride maintains osmotic pressure, gentamicin is a broad-spectrum antibiotic, has strong antibacterial activity against Pseudomonas, Proteus, coliforms, staphylococcus, aerobic bacillus and other Gram negative and negative bacteria, crystal violet mainly inhibits the growth of Gram-positive bacteria and Candida, lactose and sucrose are fermentable carbon sources, phenol red is an acid-base indicator, Burkholderia cepacia can oxidize and ferment sucrose to produce a small amount of acid, Lactose is not fermented, and the medium is slightly yellowish when grown. Usage: Weigh 36. 58g of this product, heat and boil, completely dissolve in 1000ml distilled water, divide into triangular bottles or test tubes, autoclave at 115 ℃ for 15 minutes, before use, add 1mg of filtered and sterilized gentamicin per 100ml medium, mix well, and set aside.