BCSA Agar with Polycolistin B and Gentamycin

Principle: Casein peptone and yeast extract provide the carbon source, nitrogen source and growth factor required for bacterial growth; Lactose and sucrose are fermentable sugars; Sodium chloride maintains a balanced osmotic pressure; Phenol red is used as a medium pH indicator; Crystal violet can inhibit the growth of Gram-positive bacteria, polymyxin B mainly inhibits the growth of most Gram-negative bacteria, and gentamicin is a broad-spectrum antibiotic, which has inhibitory effects on a variety of Gram-negative bacteria and positive bacteria. Agar is used as a coagulant for the medium. Usage: weigh 50. 58g of this product, heat and boil, completely dissolve in 1000ml distilled water, divide into triangular bottles, autoclave at 115 ℃ for 15 minutes, and when cooled to about 50 ℃, add filtered and sterilized polymyxin B 60000U and gentamicin 1mg per 100ml of culture medium, mix well, pour into a sterile dish, and set aside.