Ascorbic Acid (AsA) Content Fluorometric Assay Kit

Ascorbic acid (AsA) is a kind of coenzyme， free radical scavenger， electron acceptor and substrate of oxalate and tartrate biosynthesis. As the most important antioxidant in plant cells， AsA plays an important role in protecting chloroplasts from oxidative damage， and is also one of the important indicators to measure the quality of crop products. Dehydroascorbic acid (DHA) is a reversible oxidation type of AsA. In vivo， it forms an redox system with ascorbic acid and has the function of electron acceptor. AsA is oxidized to DHA by an oxidizing agent. DHA then reacts with o-phenylenediamine to form a quinoxaline derivative. Its fluorescence intensity at the excitation wavelength of 338 nm and the emission wavelength of 420 nm is proportional to the AsA content.