Alkaline Phosphatase(AKP/ALP) Activity Assay Kit

Determination of Significance: AKP/ALP is a zinc-containing glycoprotease, which hydrolysis various natural and synthetic phospholipid monoester compounds in alkaline condition. AKP / ALP are widely distributed in human organs, mainly in liver. Measurement Principle: In alkaline condition, AKP/ALP catalyzes hydrolysis disodium phenyl phosphate to phenol, and the phenol reacts with 4-Aminoantipyrine and potassium ferricyanide to form red quinone derivative, which can be detect absorbance at 510 nm. AKP/ALP activity can be calculated by measuring the absorbance increase rate at 510 nm. Self Provided: Spectrophotometer/Microplate Reader, Micro Glass Cuvette/96 Well Flat-Bottom Plate, Transferpettor, Desk Centrifuge, Ice and Distilled Water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.