ADPG Pyrophosphorylase(AGP) Activity Assay Kit

Determination of Significance: ADPG pyrophosphorylase (AGP) exists mainly in plants, is the main rate-limiting step in plant starch biosynthesis, which catalyzes the reaction of glucose-1-phosphate (G1P) with ATP to produce direct precursor adenosine diphosphate glucose (ADPG) for starch synthesis. Measurement Principle: AGP catalyzes the reverse reaction to produce G1P, the added phosphate hexose mutase and 6-phosphate glucose dehydrogenase catalyze the formation of 6-phosphate gluconate and NADPH. In this kit, the activity of AGP is determined by the increase rate of NADPH at 340 nm. Self Provided: Ultraviolet spectrophotometer, Water bath/incubator, benchtop centrifuge, adjustable pipette, 1mL quartz cuvette, mortar/homogenizer, ice, and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.