Acyl-CoA synthetase(ACS) Activity Assay kit

Acyl-CoA Synthetase ( ACS， EC 6.2.1.3 ) is a key enzyme in fatty acid metabolism， which catalyzes the binding of fatty acids to coenzyme A to generate activated acyl-CoA by consuming ATP. The enzyme is the initial step of fatty acid β-oxidation， and the activated acyl-CoA needs to enter the mitochondrial matrix through the carnitine transport system for subsequent decomposition. In the liver， activated acyl-CoA can be used as an energy metabolic substrate for β-oxidation， as well as a precursor for the synthesis of triglycerides and phospholipids. In the presence of ATP and Mg2+， Acyl Coa Synthetase ( ACS ) catalyzes the combination of FFA and coenzyme A to form acyl coenzyme A. Acyl coenzyme A is catalyzed by Acyl-CoA Oxidase ( ACO ) to generate hydrogen peroxide ( H2O2 ). H2O2 reacts with the fluorescent probe to form a strong fluorescent product under the action of the enzyme. The fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm is proportional to the content of H2O2. The kit calculates the ACS activity of the sample by measuring the rate of H2O2 formation.