Acetolactate Synthase（ALS）Activity Assay Kit

Determination of Significance: Acetolactate Synthase (ALS, EC 4.1.3.18), also known as Acetohydroxyacid synthase (AHAS). ALS is present in plant growth and is a key enzyme in the induction of the biosynthesis of the branched-chain amino acids (Ile, Leu, and Val) during the The key enzyme in the first stage of biosynthesis of branched chain amino acids (Ile, Leu, Val). Measurement Principle: ALS catalyzes the formation of acetolactate from pyruvate, which is decarboxylated under certain conditions to form acetoin, which reacts with creatine and α-naphthol under alkaline conditions to form a red substance with a maximum absorbance value at 525 nm. Self Provided: Microplate Reader/spectrophotometer, water bath/incubator, benchtop centrifuge, balance, adjustable pipettes, micro glass cuvettes/96-well plates, mortar and homogenizer, distilled water, ice, anhydrous ethanol and ammonium sulphate. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.