5'-nucleotidase（5'-NT）Activity Assay Kit

Determination of Significance: 5'-nucleotidase (5'-NT) is a kind of hydrolase with low substrate specificity, which can act on a variety of nucleotides. It widely exists in various plant, animal tissues, serum and plasma. 5'-NT is a special phosphate hydrolase, which acts on nucleoside-5'-phosphate such as AMP (adenosine-5'-phosphate or adenosine monophosphate) to produce inorganic phosphate and nucleoside. The activity of 5'-NT can be calculated by determining the content of inorganic phosphorus. Measurement Principle: 5'-nucleotidase (5'-NT) is a kind of hydrolase with low substrate specificity, which can act on a variety of nucleotides. It widely exists in various plant, animal tissues, serum and plasma. 5'-NT is a special phosphate hydrolase, which acts on nucleoside-5'-phosphate such as AMP (adenosine-5'-phosphate or adenosine monophosphate) to produce inorganic phosphate and nucleoside. The activity of 5'-NT can be calculated by determining the content of inorganic phosphorus. Self Provided: Balance, Spectrophotometer, desktop centrifuge, cryogenic centrifuge, constant temperature water bath/constant temperature incubator, 1 mL glass cuvette, transferpettor, mortar/homogenizer, ice, distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.