Solarbio

4-HNE ELISA Kit

توضیحات و پروتکل‌های فنی

This ELISA kit uses the Competitive-ELISA principle. Themicro ELISA plate provided in this kit has been pre-coated with ST/5-HT. During the reaction, ST/5-HT in samples or Standard competes with a fixed amount of ST/5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ST/5-HT. Excess conjugate and unbound sample or standard are washed from the plate, and Streptavidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of ST/5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKSM-0044-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

75min

Detection Range:

0.63-40 ng/ml

Recovery:

80%-120%

Sample Type:

Serum, plasma, Cell culture supernatant

Precision:

Coefficient of variation is < 10%

Sensitivity:

0.38 ng/ml

Specificity:

This kit recognizes 4-HNE in samples. No significant cross-reactivity or interference between 4-HNE and analogues was observed.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

120000000 تومان