β-glucosidase(β-GC) Activity Assay Kit

Determination of Significance: β-glucosidase (β-GC, EC 3.2.1.21) is an enzyme found broadly in animals, plants, microorganisms and cultured cells, which catalyzes the hydrolysis of base-glycosidic bonds and has many physiological functions. In cellulose saccharification, β-GC is responsible for further hydrolysis of cellulose disaccharides and cellulose oligosaccharides to produce glucose. β-GC hydrolyzes the aroma precursors of terpenes, making the glycoside turns from bond state to free state, thus producing the fragrance. β-GC can also hydrolyze wild sakuraside in plants and release HCN, thereby preventing insects from eating. Measurement Principle: β-GC can catalyze the p-nitrophenyl-β-D-glucopyranoside to p-nitrophenol. The p-nitrophenol has characteristic of absorption at 400 nm. In this kit, the β-GC activity is quantified by measuring the increase in the color development at 400 nm. Self Provided: Spectrophotometer/microplate reader, desk centrifuge, water bath/constant temperature incubator, sonicator, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.