β-Carotene Content HPLC Assay kit

Determination of Significance: β-carotene, one of the carotenoids, is an orange-yellow fat-soluble compound that is ubiquitous in nature and is the most stable natural pigment. Many natural foods such as green vegetables, sweet potatoes, carrots, spinach, papaya, mangoes, etc. , are rich in β-carotene. Measurement Principle: PEPCK catalyzes oxaloacetic acid to form phosphoenolpyruvate and CO2, pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+ in turn, and determine the NADH decline rate at 340 nm, which can reflect the PEPCK activity. Self Provided: Ultraviolet spectrophotometer/microplate reader, low temperature centrifuge, water bath pot, micro quartz cuvette/96 well flat-bottom plate (UV), adjustable pipette, mortar/homogenizer, ice and distilled water Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.