α-L-Arabinofuranosidase (α-L-Af) Activity Assay kit

Determination of Significance: α-L-Arabinofuranosidase (α-L-Af) belongs to the family of glycosyl hydrolases, which can be hydrolyzed from the non-reducing ends of polymers containing arabinose residues such as arabinoxylan, arabinan and arabinose galactosan etc. to form an L-arabinose molecule. This enzyme plays an important role in the degradation of hemicellulose, the ripening and softening of fruits. Measurement Principle: α-L-Af decomposes p-nitrophenyl-α-L-arabinofuranoside to produce p-nitrophenol, p-nitrophenol has a maximum absorption peak at 400 nm, which can be calculated by measuring the change in absorbance at 400 nm. Self Provided: Spectrophotometer/ Microplate reader, cryogenic centrifuge, centrifuge, water-bath/Constant temperature incubator, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.