α-Ketoglutarate Dehydrogenase(α-KGDH) Activity Assay Kit

Determination of Significance: α-Ketoglutarate Dehydrogenase (α-KGDH, EC 1.2.4.2) is one of the key enzymes in the regulation of tricarboxylic acid cycle and widely exists in mitochondria of animal, plant, microorganisms and cultured cells, which catalyzes the oxidative decarboxylation of α-ketoglutarate to succinyl coenzyme A. Measurement Principle: α-KGDH catalyzes α-ketoglutarate, NAD+ and coenzyme A to form succinyl coenzyme A, carbon dioxide and NADH. NADH has a characteristic absorption peak at 340 nm. The activity of α-KGDH is expressed by the formation rate of NADH. Self Provided: Ultraviolet spectrophotometer/microplate reader, water-bath/constant temperature incubator, tabletop centrifuge, adjustable pipette, micro quartz cuvette/96 well flat-bottom plate (UV plate), mortar/homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.